RNeasy Mini Kit

RNA isolation / purification Cells - immortalized RPMI 2650

Experiment
RNA isolation / purification Cells - immortalized RPMI 2650
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

- For purifying RNA add either 10 μl β-mercapto ethanol (β-ME) or 20 μl 2 M dithiothreitol (DTT).

Publication protocol

otal RNA was isolated using TRIzol (Invitrogen, Mulgrave, VIC, Australia) and the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) from 12 NMC cell lines (11060, 14169, 8645, RPMI2650, TC797, TY82, 10326, HCC2429, PER-403, PER-624, PER-704, P896-CL) and the P896-FB fibroblast line according to the manufacturers’ protocols, with QC performed using the Bioanalyzer 2100 (Agilent Technologies Australia, Mulgrave VIC).

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized RPMI 2650 using RNeasy Mini Kit from Qiagen.

Paper title
Molecular-genetic profiling and high-throughput drug screening in NUT midline carcinoma—an aggressive and fatal disease
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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