DNeasy Blood & Tissue Kit

DNA isolation / purification Bacteria - Gram positive Bacillus subtilis

Experiment
DNA isolation / purification Bacteria - Gram positive Bacillus subtilis
Product
DNeasy Blood & Tissue Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA

Publication protocol

Whole-genome resequencing.
Chromosomal DNA was isolated from strains W168 and HB13506 grown in LB medium to an optical density at 600 nm (OD600) of 0.4 by using the Qiagen DNeasy blood and tissue kit. The quantity and purity of DNA were determined using a NanoDrop spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE), and DNA was sequenced and analyzed by the Cornell University Life Sciences Core Laboratories Center using Illumina DNA sequencing. The sequence data were assembled with MOSAIC by using the reference sequence (35) under GenBank accession number ABQK00000000.

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram positive Bacillus subtilis using DNeasy Blood & Tissue Kit from Qiagen.

Paper title
A Mutation of the RNA Polymerase β′ Subunit () Confers Cephalosporin Resistance in
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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download PDF Download manufacturer protocol

Videos

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