DNeasy Blood & Tissue Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C - Use prewarmed TE buffer to elute the DNA

Publication protocol

Whole-genome resequencing.
Chromosomal DNA was isolated from strains W168 and HB13506 grown in LB medium to an optical density at 600 nm (OD600) of 0.4 by using the Qiagen DNeasy blood and tissue kit. The quantity and purity of DNA were determined using a NanoDrop spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE), and DNA was sequenced and analyzed by the Cornell University Life Sciences Core Laboratories Center using Illumina DNA sequencing. The sequence data were assembled with MOSAIC by using the reference sequence (35) under GenBank accession number ABQK00000000.

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy Blood & Tissue Kit below.

Download manufacturer protocol

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