RNeasy Mini Kit

RNA isolation / purification Cells - immortalized OVCAR-3

Experiment
RNA isolation / purification Cells - immortalized OVCAR-3
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

- For purifying RNA add either 10 μl β-mercapto ethanol (β-ME) or 20 μl 2 M dithiothreitol (DTT).

Publication protocol

Real-time PCR was performed to quantitatively evaluate the P14 gene expression after the treatment of cell groups with ATRA, GEN, and their combinations. Total RNA was isolated by RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol and in then treated with RNase free DNase (Qiagen, Hilden, Germany) to eliminate the genomic DNA.

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized OVCAR-3 using RNeasy Mini Kit from Qiagen.

Paper title
All-trans retinoic acid and genistein induce cell apoptosis in OVCAR-3 cells by increasing the P14 tumor suppressor gene
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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