TRI Reagent® Sigma

RNA isolation / purification Cells - immortalized Rael

Experiment
RNA isolation / purification Cells - immortalized Rael
Product
TRI Reagent® Sigma from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA.

- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Total RNA was isolated from the cells using TRI Reagent (Sigma), followed by DNase I treatment of 1 µg RNA and reverse transcription with Transcriptor High Fidelity cDNA Synthesis Kit (Roche) using lamin A/C (LMN-RT) and GAPDH-specific (GAPDH-RT) oligonucleotides

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

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