NucleoSpin® RNA

RNA isolation / purification Tissue - Human Adipose

Experiment

RNA isolation / purification Tissue - Human Adipose

Product

NucleoSpin® RNA from Macherey Nagel

Manufacturer

Macherey Nagel

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
Aliquot rDNase and store at -20 °C.	Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Upstream tips
Aliquot rDNase and store at -20 °C.
Protocol tips
Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

Total RNA was isolated from inguinal subcutaneous WAT and subscapular BAT using NucleoSpin RNA II kit (Macherey and Nagel). Synthesis of cDNA was performed using SuperScript® III reverse transcriptase (Invitrogen). Quantitative real-time PCR reactions for indicated genes were conducted on a 7900HT sequence detection system (Applied Biosystems) using TaqMan Assay-on-Demand primer sets

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Discussion

5 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Adipose using NucleoSpin® RNA from Macherey Nagel.

Paper title

Endocannabinoid regulation in white and brown adipose tissue following thermogenic activation

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Manufacturer protocol

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Videos

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