RNeasy Micro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.		Always blank the spectrophotometer with your elution buffer. When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Upstream tips
This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
Always blank the spectrophotometer with your elution buffer. When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

Total RNA extraction from blood/cultured cells and tissue was performed with a spin column-based PAXgene Blood RNA Kit/RNeasy Micro Kit (Qiagen, Hilden, Germany) and phenol-based kit (TRIzol; Invitrogen, Carlsbad, CA, USA). RNA samples were purified by exposure to DNAse I enzyme to digest genomic DNA. High RNA purity was confirmed via microfluidic-based electrophoresis (Agilent 2100 Bioanalyzer platform; Agilent Technologies, Santa Clara, CA, USA).

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

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