TRIzol Reagent

RNA isolation / purification Tissue - Human Artery / Aorta

Experiment

RNA isolation / purification Tissue - Human Artery / Aorta

Product

TRIzol Reagent from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

otal RNA was extracted from the tissue samples (50-100 mg tissue) using the TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s protocol. Then RNA degradation and contamination was detected by 1% agarose gels. RNA integrity and concentration was assessed by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The samples were preserved at -80 °C for further analysis.

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Artery / Aorta using TRIzol Reagent from Thermo Fisher Scientific.

Paper title

LncRNA Expression Profile of Human Thoracic Aortic Dissection by High-Throughput Sequencing.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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