High Pure miRNA Isolation Kit
RNA isolation / purification Tissue - Human Bone marrow
Protocol tips
| Protocol tips |
|---|
| - To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step |
Publication protocol
The Roche High Pure miRNA Isolation Kit (Catalogue #05080576001, Dee Why, NSW AUST) was used as per the manufacturer’s instructions where possible, however modifications to the protocol were needed to improve the total RNA yieldFull paper Login or join for free to view the full paper.
Reviews
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Sigma-Aldrich for High Pure miRNA Isolation Kit below.
Download manufacturer protocol
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