RNeasy Micro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.		- Always blank the spectrophotometer with your elution buffer. - When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer. - When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

total RNA was isolated using the RNeasy Micro Kit (Qiagen, Valencia, CA) and treated with the Turbo DNA-freeTM Kit (Life Technologies, Grand Island, NY) to remove potential contaminating DNA that may lead to false-positive amplification. RNA quality and purity was confirmed with a Nanodrop spectrophotometer (Thermo Scientific). The resulting intact RNA was used for either RNAseq analysis or QPCR analyses (see below). RNAseq was performed using RNA from the bone samples, using methods as extensively described previously from our laboratory. The only exception to this was that because ample total RNA was obtained from the bone biopsies, there was no need to amplify the mRNA species

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

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