GenElute™ Bacterial Genomic DNA Kit

DNA isolation / purification Bacteria - Gram negative E.coli

Experiment
DNA isolation / purification Bacteria - Gram negative E.coli
Product
GenElute™ Bacterial Genomic DNA Kit from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
Examine reagents for precipitation. If any reagent forms a precipitate, warm at 55–65 °C until the precipitate dissolves and cool to room temperature before use.

Publication protocol

Genomic analysis
Bacterial DNA was extracted from 1-day old cultures of selected strains using GenElute™ bacterial genome kit (Sigma Aldrich®, USA). The genome sequences were generated on an Illumina MiSeq using v3 chemistry and 300 bp paired end reads using dual indexed Nextera XT libraries. The de novo assembly was performed using SPAdes assembly program and Quast [32]. Genome annotation used the SEED-based automated annotation system provided by the RAST server (http://rast.nmpdr.org) and prokaryotic genome annotation system PROKKA [33].

Full paper   Login or join for free to view the full paper.

Reviews

GenElute™ Bacterial Genomic DNA Kit from Sigma-Aldrich has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Bacteria - Gram negative E.coli using GenElute™ Bacterial Genomic DNA Kit from Sigma-Aldrich.

Paper title
The molecular characterisation of K1 isolated from neonatal nasogastric feeding tubes
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for GenElute™ Bacterial Genomic DNA Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Bacteria - Gram negative E.coli using GenElute™ Bacterial Genomic DNA Kit from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms