SV Total RNA Isolation System
RNA isolation / purification Tissue - Human Breast
Publication protocol
RNA was isolated using the SV Total RNA isolation kit (Promega). Verso cDNA synthesis kit and Absolute Blue SYBR Green ROX mix (Thermo Fisher Scientific Inc.) were used for RT-PCR. Real-time PCR (Rotor Gene 3000, Corbett) included amplification cycles of 5 sec at 95°c followed by 30 sec at 60°c.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
4 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Manufacturer protocol
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