MagNA Pure Compact RNA Isolation Kit
RNA isolation / purification Tissue - Human Bronchi
Publication protocol
The uMS pellet and tissue from the core were used to obtain DNA or RNA. These samples were homogenized with a MagNA Lyser for 60 seconds at 6500 rpm and placed on a cooling block for 1 minute. Supernatants (100 μl) were used for DNA extraction with a MagNA Pure Compact Nucleic Acid Isolation Kit. Supernatants (350 μl) from homogenates were centrifuged and RNA was extracted using a MagNA Pure Compact RNA Isolation Kit. cDNA cloning was performed with a First Strand cDNA Synthesis Kit (Roche). Extracted DNA and cDNA were stored at 4°C.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Roche Lifesciences for MagNA Pure Compact RNA Isolation Kit below.
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