MagNA Pure Compact RNA Isolation Kit

RNA isolation / purification Tissue - Human Bronchi

Experiment
RNA isolation / purification Tissue - Human Bronchi
Product
MagNA Pure Compact RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 1x10^6 cells

Publication protocol

The uMS pellet and tissue from the core were used to obtain DNA or RNA. These samples were homogenized with a MagNA Lyser for 60 seconds at 6500 rpm and placed on a cooling block for 1 minute. Supernatants (100 μl) were used for DNA extraction with a MagNA Pure Compact Nucleic Acid Isolation Kit. Supernatants (350 μl) from homogenates were centrifuged and RNA was extracted using a MagNA Pure Compact RNA Isolation Kit. cDNA cloning was performed with a First Strand cDNA Synthesis Kit (Roche). Extracted DNA and cDNA were stored at 4°C.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Bronchi using MagNA Pure Compact RNA Isolation Kit from Roche Lifesciences.

Paper title
Transbronchial biopsy needle rinse solution used for comprehensive biomarker testing in patients with lung cancer.
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for MagNA Pure Compact RNA Isolation Kit below.

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