NucleoSpin® RNA/Protein

RNA isolation / purification Tissue - Human Cornea

Experiment

RNA isolation / purification Tissue - Human Cornea

Product

NucleoSpin® RNA/Protein from Macherey Nagel

Manufacturer

Macherey Nagel

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit is designed for tissue samples of 30 mg	- For high yield and high concentration: Elute with the standard elution volume and apply the eluate once more onto the column for reelution.

Upstream tips
- This kit is designed for tissue samples of 30 mg
Protocol tips
- For high yield and high concentration: Elute with the standard elution volume and apply the eluate once more onto the column for reelution.

Publication protocol

Total RNA was extracted using mirVana miRNA isolation kit from ThermoFisher Scientific, and with NucleoSpin RNA/Protein isolation kit (MACHEREY-NAGEL, Inc., Bethlehem, PA, USA), according to the manufacturer's recommendations. RNA quality was evaluated using Agilent 2100 Bioanalyzer with Agilent's RNA 6000 Pico Kit (Agilent, Santa Clara, CA, USA). We selected RNA samples with a minimum RIN (RNA Integrity Number) score of 6 for RNA-Seq.

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Discussion

5 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Cornea using NucleoSpin® RNA/Protein from Macherey Nagel.

Paper title

Differential Expression of Coding and Long Noncoding RNAs in Keratoconus-Affected Corneas

Full paper

Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA/Protein below.

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Videos

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