RNeasy Mini Kit

RNA isolation / purification Tissue - Human Decidua

Experiment

RNA isolation / purification Tissue - Human Decidua

Product

RNeasy Mini Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted from decidua samples using TRI Reagent and RNeasy mini kit (Qiagen, Crawley, UK) as per the manufacturer's guidelines. The quantity and quality of RNA was assessed using a Nano-Drop ND 1000 spectrophotometer (Thermo Scientific, UK). In preparation for the microarray experiment, 750ng total RNA was amplified and biotin-labelled using the Illumina TotalPrep RNA Amplification Kit (Ambion, UK). The quantity and quality of the biotin-labelled cRNA samples was further assessed by the Wellcome Trust Clinical Research Facility, Edinburgh using a Bioanalyzer 2100, according to their protocols (Agilent Technologies LDA UK Limited, Cheshire, UK).

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Discussion

5 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Decidua using RNeasy Mini Kit from Qiagen.

Paper title

Immune cell and transcriptomic analysis of the human decidua in term and preterm parturition

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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Videos

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