miRNeasy Mini kit
RNA isolation / purification Tissue - Human Duodenum
Protocol tips
| Protocol tips |
|---|
| - To capture a higher amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step. |
Publication protocol
Total RNA was extracted from biopsies using a MiRcury RNA Isolation Kit (Exiqon, Vedbaek, Denmark) following the manufacturer’s instructions. Total RNA extraction from plasma was performed using miRNeasy Serum/Plasma Kit (Qiagen, Valencia, CA) and following the manufacturer’s instructions. Quality and quantification of extracted RNA was performed by NanoDrop 1000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA).Full paper Login or join for free to view the full paper.
Reviews
miRNeasy Mini kit from Qiagen has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Discussion
5 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Duodenum using miRNeasy Mini kit from Qiagen.
Manufacturer protocol
Download the product protocol from Qiagen for miRNeasy Mini kit below.
Download manufacturer protocol
Videos
Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human Duodenum using miRNeasy Mini kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Outsource your experiment
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment