miRNeasy Mini kit
RNA isolation / purification Tissue - Human Duodenum
Protocol tips
| Protocol tips |
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| - To capture a higher amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step. |
Publication protocol
Total RNA was extracted from biopsies using a MiRcury RNA Isolation Kit (Exiqon, Vedbaek, Denmark) following the manufacturer’s instructions. Total RNA extraction from plasma was performed using miRNeasy Serum/Plasma Kit (Qiagen, Valencia, CA) and following the manufacturer’s instructions. Quality and quantification of extracted RNA was performed by NanoDrop 1000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Qiagen for miRNeasy Mini kit below.
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