NucleoSpin® RNA

RNA isolation / purification Tissue - Human Colon

Experiment
RNA isolation / purification Tissue - Human Colon
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

DNA was purified from FFPE samples using QIAamp DNA FFPE Tissue (QIAGEN) and Nucleospin FFPE DNA (Machery-Nagel) kits; RNA was purified from FFPE samples using miRNeasy FFPE (QIAGEN), Nucleospin FFPE RNA (Machery-Nagel) and ExpressArt FFPE RNAready (Amp Tec) kits. In order to test parallel purification of RNA and DNA from the same FFPE sections, the Nucleospin FFPE RNA/DNA kit (Machery-Nagel) and RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Ambion) were included

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Reviews

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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

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