PAXgene Tissue RNA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Starting material for RNA purification should be up to 10 mg of a tissue sample. - Add 10 µl β-ME per 1 ml Buffer TR1 before use. - To increase RNA yield and to reduce formalin contamination, increase lysate digestion with proteinase K from 15 min at 45°C to 6 hours at 45°C	- Do not overload the PAXgene RNA MinElute spin column, as this will significantly reduce RNA yield and quality	- RNA concentration must be measured in 10 mM Tris·Cl, pH 7.5* for accurate quantification

Upstream tips
- Starting material for RNA purification should be up to 10 mg of a tissue sample. - Add 10 µl β-ME per 1 ml Buffer TR1 before use. - To increase RNA yield and to reduce formalin contamination, increase lysate digestion with proteinase K from 15 min at 45°C to 6 hours at 45°C
Protocol tips
- Do not overload the PAXgene RNA MinElute spin column, as this will significantly reduce RNA yield and quality
Downstream tips
- RNA concentration must be measured in 10 mM Tris·Cl, pH 7.5* for accurate quantification

Publication protocol

Tissue samples were homogenized in the MagNA Lyser (Hoffmann-La Roche). AllPrep DNA/RNA mini kit (Qiagen) was used to isolate nucleic acids from the samples. RNA from tissues collected in PAXgene containers was extracted using the PAXgene Tissue RNA Kit (PreAnalytiX) according to the manufacturer’s instruction

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Manufacturer protocol

Download the product protocol from PreAnalytiX for PAXgene Tissue RNA Kit below.

Download manufacturer protocol

Videos

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