PAXgene Tissue RNA Kit

RNA isolation / purification Tissue - Human Colon

Experiment
RNA isolation / purification Tissue - Human Colon
Product
PAXgene Tissue RNA Kit from PreAnalytiX
Manufacturer
PreAnalytiX

Protocol tips

Upstream tips
- Starting material for RNA purification should be up to 10 mg of a tissue sample.

- Add 10 µl β-ME per 1 ml Buffer TR1 before use.

- To increase RNA yield and to reduce formalin contamination, increase lysate digestion with proteinase K from 15 min at 45°C to 6 hours at 45°C
Protocol tips
- Do not overload the PAXgene RNA MinElute spin column, as this will significantly reduce RNA yield and quality
Downstream tips
- RNA concentration must be measured in 10 mM Tris·Cl, pH 7.5* for accurate quantification

Publication protocol

Tissue samples were homogenized in the MagNA Lyser (Hoffmann-La Roche). AllPrep DNA/RNA mini kit (Qiagen) was used to isolate nucleic acids from the samples. RNA from tissues collected in PAXgene containers was extracted using the PAXgene Tissue RNA Kit (PreAnalytiX) according to the manufacturer’s instruction

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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Colon using PAXgene Tissue RNA Kit from PreAnalytiX.

Paper title
The focus on sample quality: Influence of colon tissue collection on reliability of qPCR data
Full paper
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Manufacturer protocol

Download the product protocol from PreAnalytiX for PAXgene Tissue RNA Kit below.

Download PDF Download manufacturer protocol

Videos

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