TRI Reagent® Sigma

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- The chloroform used for phase separation should not contain isoamyl alcohol or other additives. - 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA

Upstream tips
- The chloroform used for phase separation should not contain isoamyl alcohol or other additives. - 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA

Publication protocol

Total RNA samples were isolated using TRI Reagent (Sigma, St. Louis, MO, USA) from the obtained fresh frozen tissue and the concentration and quality for all obtained samples were analyzed using Nanodrop and Agilent Bioanalyzer, respectfully. GeneChip analysis was performed on EOC samples to identify genes correlating to FOLR1 expression. The Affymetrix Exon Array ST 1.0 using the Ambion WT Expression kit was used as directed with 100 ng of total RNA. Array data was normalized using standard RMA Refiner workflow in GeneData. A similarity search to find those genes that show high correlation (Positive or Negative) in ovarian samples from Proteogenex was performed in GeneData Analyst using search by distance with a similarity measurement of correlation. A total of 27 genes had +/− correlation greater than 0.8.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

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