TRI Reagent® Sigma

RNA isolation / purification Tissue - Human Fallopian tubes

Experiment

RNA isolation / purification Tissue - Human Fallopian tubes

Product

TRI Reagent® Sigma from Sigma-Aldrich

Manufacturer

Sigma-Aldrich

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
- The chloroform used for phase separation should not contain isoamyl alcohol or other additives. - 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA

Upstream tips
- The chloroform used for phase separation should not contain isoamyl alcohol or other additives. - 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA

Publication protocol

Total RNA samples were isolated using TRI Reagent (Sigma, St. Louis, MO, USA) from the obtained fresh frozen tissue and the concentration and quality for all obtained samples were analyzed using Nanodrop and Agilent Bioanalyzer, respectfully. GeneChip analysis was performed on EOC samples to identify genes correlating to FOLR1 expression. The Affymetrix Exon Array ST 1.0 using the Ambion WT Expression kit was used as directed with 100 ng of total RNA. Array data was normalized using standard RMA Refiner workflow in GeneData. A similarity search to find those genes that show high correlation (Positive or Negative) in ovarian samples from Proteogenex was performed in GeneData Analyst using search by distance with a similarity measurement of correlation. A total of 27 genes had +/− correlation greater than 0.8.

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Fallopian tubes using TRI Reagent® Sigma from Sigma-Aldrich.

Paper title

Gene Expression Analyses Support Fallopian Tube Epithelium as the Cell of Origin of Epithelial Ovarian Cancer

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

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