RNeasy Plus Micro Kit

RNA isolation / purification Tissue - Human Gut

Experiment

RNA isolation / purification Tissue - Human Gut

Product

RNeasy Plus Micro Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.		- Always blank the spectrophotometer with your elution buffer. - When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells, or up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer. - When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

Total RNA was isolated using RNeasy® Plus Micro Kit (Qiagen). Maximal amounts of RNA were encoded by reverse transcriptase (Platinum® Taq DNA Polymerase, Invitrogen, Karlsruhe, Germany) and gene expression analysis conducted.

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Reviews

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Gut using RNeasy Plus Micro Kit from Qiagen.

Paper title

Age-related gene expression analysis in enteric ganglia of human colon after laser microdissection

Full paper

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Micro Kit below.

Download manufacturer protocol

Videos

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