RNeasy Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Brain samples were homogenized in Trizol and total RNA was isolated from cells using the RNeasy Kit (Qiagen) according to the manufacturer's protocol. An additional DNase1 digestion step was performed to ensure that the samples were not contaminated with genomic DNA. RNA purity was checked by calculating RIN in Agilent BioAnalyzer 2100 (Agilent, Santa Clara, CA). Transcriptome sequencing libraries were prepared using the TruSeq RNA Access Library Prep Kit® (Illumina, CA; US). Paired end sequencing (100 bp) was performed at an Illumina HiSeq 2500 instrument. All the 100 bp paired end raw reads were quality checked for low quality bases and adapter sequences (Processing of raw reads). Quality check was done using NGS QC Toolkit v2.3.1 [58]. Low quality bases and reads were filtered out from the datasets prior to read mapping. We used Tophat RNA aligner to map sequence read to UCSC (the University of California Santa) reference human genome Hg19

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Hippocampus using RNeasy Mini Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human Hippocampus using RNeasy Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.