MagMAX™-96 Total RNA Isolation Kit

RNA isolation / purification Tissue - Human ileum

Experiment

RNA isolation / purification Tissue - Human ileum

Product

MagMAX™-96 Total RNA Isolation Kit from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit can be used for the isolation of RNA from ≤ 5 mg animal tissue.	- To avoid DNA contamination, either reduce sample size, or include an additional DNase treatment step after RNA isolation	- If there is low yield and/or inconsistent yield, lowering the amount of sample input may improve results

Upstream tips
- This kit can be used for the isolation of RNA from ≤ 5 mg animal tissue.
Protocol tips
- To avoid DNA contamination, either reduce sample size, or include an additional DNase treatment step after RNA isolation
Downstream tips
- If there is low yield and/or inconsistent yield, lowering the amount of sample input may improve results

Publication protocol

RNA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems), RNA isolation kits, and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). A total of 26 samples were sequenced for 50-bp single-end reads across 10 lanes on an Illumina HiSeq 2500 by the University of Michigan DNA Sequencing Core. All reads were quantified using Kallisto pseudo alignment to an index of transcripts from all human genes within the Ensembl GRCh38 database.

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Reviews

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MagMAX™-96 Total RNA Isolation Kit below.

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Videos

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