MagNA Pure LC RNA Isolation Kit III

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- The MagNA Pure LC 2.0 Instrument, together with its software is required for analysing the yields of this kit. - Do not use more sample than this kit is designed to handle. - Do not allow Wash Buffer I or the Lysis/Binding Buffer to mix with sodium hypochlorite (bleach) solution. This mixture can produce a highly toxic gas.

Upstream tips
- The MagNA Pure LC 2.0 Instrument, together with its software is required for analysing the yields of this kit. - Do not use more sample than this kit is designed to handle. - Do not allow Wash Buffer I or the Lysis/Binding Buffer to mix with sodium hypochlorite (bleach) solution. This mixture can produce a highly toxic gas.

Publication protocol

The biopsies were homogenized using the MagNA Lyser instrument and MagNa Lyser Green Beads tubes (Roche Applied Science, Mannheim, Germany). Total RNA was extracted from 10 mg of human fresh-frozen intestinal biopsies using the MagNA Pure LC RNA Isolation Kit III (Tissue) and the MagNA Pure LC instrument (Roche), and eluted into RNAse-free water. To generate complementary DNA (cDNA), 2 μg total RNA was subjected to reverse transcriptase-PCR.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human ileum using MagNA Pure LC RNA Isolation Kit III from Roche Lifesciences.

Manufacturer protocol

Download the product protocol from Roche Lifesciences for MagNA Pure LC RNA Isolation Kit III below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human ileum using MagNA Pure LC RNA Isolation Kit III from Roche Lifesciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.