RNeasy Fibrous Tissue Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit purifies RNA from up to 30 mg tissue		- If there is low RNA yield,Repeat RNA elution, incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - If there is low Low A260/A280 value use 10 mM Tris·Cl, pH 7.5, not RNase-free water, to dilute the sample before measuring purity. - If RNA does not perform well in downstream experiments this could be due to salt carryover dyuring elution step, so ensure that Buffer RPE is at 15–25°C.

Upstream tips
- This kit purifies RNA from up to 30 mg tissue
Downstream tips
- If there is low RNA yield,Repeat RNA elution, incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - If there is low Low A260/A280 value use 10 mM Tris·Cl, pH 7.5, not RNase-free water, to dilute the sample before measuring purity. - If RNA does not perform well in downstream experiments this could be due to salt carryover dyuring elution step, so ensure that Buffer RPE is at 15–25°C.

Publication protocol

Non-frozen and thawed synovial biopsies were cultured for 24 hours and subsequently snap-frozen in liquid nitrogen. Total RNA was isolated using the RNeasy fibrous tissue kit (Qiagen, Venlo, The Netherlands). Frozen synovial samples were transferred to MagNA Lyser Green Beads tubes (Roche) containing RLT lysis buffer. The tissue was disrupted in the MagNA lyser for 3 x 20 sec at 6000 rpm with one minute cooling between cycles. The remainder of the procedure was performed according to manufacturer instructions with on column DNA digestion.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Fibrous Tissue Mini Kit below.

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