TRIzol Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was also extracted from mouse kidneys and HEK-293 cell lysates using TRIzol® and the RNAeasy Mini Kit (QIAGEN), respectively. hsa-miR-422a was quantified with TaqMan Micro-RNA Assay (Applied Biosystems) using RNU48 as reference gene. mmu-miR-378 (mmu-miR-378a-3p), which is the mouse paralog of hsa-miR-422a, was quantified with TaqMan Micro-RNA Assay (Applied Biosystems) using U6 snRNA for normalization. For mRNA quantification, reverse transcription was performed with the PrimeScript 1st strand cDNA Synthesis Kit (TAKARA). Human and mouse KLK4 was measured with TaqMan Assay (Applied Biosystems) using GAPDH as reference gene. CT values were normalized to endogenous control and the fold-change relative to a control sample (2ΔΔCT) was used for relative expression analysis

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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