MEDOX-Easy Total RNA Extraction Reagent
RNA isolation / purification Tissue - Human Mouth
Publication protocol
The tissue samples were immersed in an RNAase inhibitor containing solution (RNA later, Invitrogen, Bangalore, India) and homogenized immediately. Total RNA was obtained from the homogenates using Trizol total RNA extraction kit (Medox, Chennai, Tamil Nadu, India). The first step in real time reverse transcription polymerase chain reaction (q-PCR) was the conversion of the total RNA to a complementary cDNA which was then carried out using a high capacity cDNA reverse transcription kit (Applied Biosystems, Invitrogen BioServices, Bangalore, India). The quantitative real-time amplification was done using a SYBR Green containing master mix kit (Qiagen, Germany) to evaluate the mRNA expression of T-bet and GATA-3 in periodontal health and disease.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Manufacturer protocol
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