TRI Reagent® Sigma

Protocol tips

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- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Skeletal muscle tissue (~30 mg) was immersed in Tri-Reagent (Sigma-Aldrich, MO, United States) in MagNA Lyser 1.4 mm beaded tubes (MagNA Lyser Green Beads, Roche, Germany) and homogenised for 40 secs at 6 m/s in a MagNA Lyser Homogeniser (Roche, Germany), before being stored on ice for 5 mins. This step was repeated three times to ensure complete disruption of muscle tissue sample. RNA was extracted using standard Tri-Reagent procedure via chloroform/isopropanol extractions and 75% ethanol washing as per manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

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