PicoPure™ RNA Isolation Kit
RNA isolation / purification Tissue - Human Nose
Publication protocol
The tissue was transferred to the lysis buffer from the Arcturus PicoPure RNA extraction kit (ThermoFisher Scientific) for cDNA synthesis, and then to Trizol (Invitrogen) for homogenisation and total RNA extraction according to the manufacturer's instructions. Single‐strand cDNA was generated using Invitrogen's Superscript III First‐Strand Synthesis System kit.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Thermo Fisher Scientific for PicoPure™ RNA Isolation Kit below.
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