MagMAX™-96 Total RNA Isolation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit can be used for the isolation of RNA from ≤ 5 mg animal tissue.	- To avoid DNA contamination, either reduce sample size, or include an additional DNase treatment step after RNA isolation	- If there is low yield and/or inconsistent yield, lowering the amount of sample input may improve results

Upstream tips
- This kit can be used for the isolation of RNA from ≤ 5 mg animal tissue.
Protocol tips
- To avoid DNA contamination, either reduce sample size, or include an additional DNase treatment step after RNA isolation
Downstream tips
- If there is low yield and/or inconsistent yield, lowering the amount of sample input may improve results

Publication protocol

Total RNA was isolated from the ovarian specimen and from theca-interstitial cells using the MagMAX-96 Total RNA Isolation Kit (Applied Biosystems) and the King Fisher robot (Thermo Scientific). Reverse transcription of total RNA to complementary DNA (cDNA) was performed using the High Capacity cDNA Reverse Transcription Kit for reverse transcription–polymerase chain reaction (RT-PCR) (Applied Biosystems). The PCR assays were set up in 28-µL volumes, consisting of 5 µL cDNA, 4.5 µL forward and 4.5 µL reverse 900n Mprimers, and 14 µL of 2× SYBR Green PCR Master Mix (Applied Biosystems).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MagMAX™-96 Total RNA Isolation Kit below.

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