Cellular Senescence Staining Kit

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	fix cells with fixing solution at RT for 5 min. Wash cells and incubate in working solution for 4 h at 37 °C

Protocol tips
fix cells with fixing solution at RT for 5 min. Wash cells and incubate in working solution for 4 h at 37 °C

Publication protocol

The Cellular Senescence Assay Kit (Cat no CBA-230, Cell Biolabs, CA, USA) was used to stain β-galactosidase-positive cells. Cells were washed twice with PBS and fixed with fixing solution at RT for 5 min. Then, the cells were washed with PBS three times and further incubated in working solution for 4 h at 37 °C. Cells were washed with PBS, and blue staining was then observed under a light microscope

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