TRIzol Reagent
RNA isolation / purification Tissue - Human Pharynx
Protocol tips
| Protocol tips |
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| - For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
Publication protocol
Tissue specimens that had been stored in liquid nitrogen were used for RNA extraction. To each 50 mg tissue sample, 1 mL TrizoL reagent (Invitrogen) homogenate was added to extract total RNA according to manufacturer’s instructions. Total RNA was assessed for purity and quantity with an ultraviolet spectrophotometer and 1% agarose gel electrophoresis. Reverse transcription was performed in accordance with instructions in the TaKaRa PrimeScriptTM RT-PCR Kit (Code No. DR014A, TaKaRa).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Manufacturer protocol
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