MagNA Pure Compact RNA Isolation Kit
RNA isolation / purification Tissue - Human Placenta
Publication protocol
Disruption and homogenization of tissue specimens were performed in lysis buffer using the MagNa Lyser Instrument (Roche Applied Science, Germany), according to the manufacturer's instructions. Isolation of total RNA was performed using the MagNa Pure Compact RNA isolation kit and the MagNa Pure Compact Instrument (Roche Applied Science, Germany). RNA was quantified by measuring absorbance at 260 nm, and RNA purity was determined by the ratios OD260 nm/280 nm and OD230 nm/280 nm using the NanoDrop instrument (NanoDrop® ND-1000, Wilmington, USA). The RNA integrity was determined by electrophoresis using the Agilent 2100 Bioanalyser (Matriks, Norway). Only samples with RNA Integrity Number (RIN) >7.2 were used for microarray.Full paper Login or join for free to view the full paper.
Reviews
MagNA Pure Compact RNA Isolation Kit from Roche Lifesciences has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Placenta using MagNA Pure Compact RNA Isolation Kit from Roche Lifesciences.
Manufacturer protocol
Download the product protocol from Roche Lifesciences for MagNA Pure Compact RNA Isolation Kit below.
Download manufacturer protocol
Videos
Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human Placenta using MagNA Pure Compact RNA Isolation Kit from Roche Lifesciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Outsource your experiment
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment