NucleoSpin® RNA
RNA isolation / purification Tissue - Human Placenta
Protocol tips
| Upstream tips |
|---|
| - Aliquot rDNase and store at -20 °C. |
| Protocol tips |
| - Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer. |
Publication protocol
Infected and mock-infected decidual and chorionic villus tissues were washed and stored at −80°C until assayed. DNA-free RNA was extracted (NucleoSpin RNA isolation kit; Macherey-Nagel) and subjected to reverse transcription (GoScript; Promega), followed by quantitative real-time PCR (7900HT; Applied Biosystems)Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
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