miRNeasy FFPE Kit

RNA isolation / purification Tissue - Human Prostate

RNA isolation / purification Tissue - Human Prostate
miRNeasy FFPE Kit from Qiagen

Protocol tips

Upstream tips
- To allow complete penetration by formalin, use tissue samples less than 5 mm thick
Protocol tips
- Perform all centrifugation steps using a microcentrifuge placed at 15–25°C
Downstream tips
- Do not exceed fixation time of 24 hours as it results in poor performance in downstream assay

Publication protocol

For RNA extraction, our results are in agreement with Boeckx et al. who reported that the RNeasy FFPE Isolation Kit (QIAGEN) performed well and was superior to the NucleoSpin FFPE RNA Isolation Kit (Macherey-Nagel) [29]. Most of the published studies that included isolation of FFPE specimen nucleic acids did not test different isolation strategies. This may partly explain some of the variation in the results reported. It is evident from both academic and commercial publications that new nucleic acid extraction methods for FFPE specimens are under continued development. Future studies should evaluate these systematically, especially with regard to their ability to counteract the detrimental effects of fixation, tissue block preparation and storage.

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4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?


5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy FFPE Kit below.

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