PicoPure™ RNA Isolation Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Can be used when there is a very less amount of starting material

Upstream tips
- Can be used when there is a very less amount of starting material

Publication protocol

Total RNA was extracted from LCM captured material using the Arcturus® PicoPure® RNA Isolation Kit (Life Technologies, Grand Island, NY) according to the manufacturer’s protocol. Following tissue dissection, the LCM caps were incubated for 30 min at 42°C in 50 μl of extraction buffer and stored at -20°C until ready for RNA extraction. Cellular lysates from successive slides were pooled into a single tube and mixed with 1 volume of ethanol (70%). The lysate/ethanol solution was loaded onto a high recovery MiraCol™ column and centrifuged at 1000 xg for 2 min followed by 16,000 xg for 30 sec. RNA was recovered in an 11 μl volume. RNA concentration and quality were measured using an Agilent RNA 6000 Pico chip with the Bioanalyzer 2100 (Agilent Technologies) using the manufacturer’s instructions. All samples used for qRT-PCR displayed RNA of good quality as assessed by its RNA Integrity Number (RIN) and of sufficient quantity to not require a pre amplification step for subsequent PCR analysis

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Prostate using PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PicoPure™ RNA Isolation Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human Prostate using PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.