RNeasy Mini Kit

RNA isolation / purification Tissue - Human Salivary glands

Experiment
RNA isolation / purification Tissue - Human Salivary glands
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted using the Qiagen RNeasy Minikit. For cDNA generation, 1 µg of total RNA was reverse transcribed using 0.5 µg oligo(dT)15–18 primers, 0.5 mM dNTPs, 1 × First‐strand Buffer, 0.01 M dithiothreitol, 400 U RnaseOut, and 200 U of M‐MLV Reverse Transcriptase (all Invitrogen), in a total volume of 20 µL per reaction. Quantitative polymerase chain reaction (Bio‐Rad, Hercules, CA, http://www.bio-rad.com) (qPCR) was performed using Bio‐Rad iQ SYBR Green Supermix according to manufacturer's instruction

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Salivary glands using RNeasy Mini Kit from Qiagen.

Paper title
Human Salivary Gland Stem Cells Functionally Restore Radiation Damaged Salivary Glands.
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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