TRIzol Reagent
RNA isolation / purification Tissue - Human Seminal vesicles
Protocol tips
| Protocol tips |
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| - For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
Publication protocol
To verify the specificity of VASA IC in germ cells, bladder and seminal vesicle epithelium high quality total RNA was extracted from normal testis-parenchyma (n = 10), semen of men with normospermia (n = 8), NOA (n = 5), OA (n = 6), bladder (n = 3), and seminal vesicles (n = 10) using TRIzol Reagent (Life Technologies, Bleiswijk, the Netherlands) according to the manufacturer's instructions. qRT-PCR was performed in duplicate using mRNA probes VASA hs00987125_m1 and endogenous control hypoxanthine-guanine phosphoribosyltransferase (HPRT)1 (4333768F; Life Technologies). Relative VASA transcript abundance was calculated using the 2-dCt method (VASA mRNA = mean Ct HPRT – mean Ct VASA)Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Manufacturer protocol
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