NucleoSpin® RNA XS

RNA isolation / purification Tissue - Human Skin

Experiment

RNA isolation / purification Tissue - Human Skin

Product

NucleoSpin® RNA XS from Macherey Nagel

Manufacturer

Macherey Nagel

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit is for the isolation of RNA from very small samples (up to 5 mg)	- For adsorption measurement, use 5 mM Tris pH 8.5 as diluent	- Do not let the flow-through touch the column outlet after the second Buffer RA3 wash. Be sure to centrifuge at the corresponding speed for the respective time in order to remove ethanolic Buffer RA3 completely.

Upstream tips
- This kit is for the isolation of RNA from very small samples (up to 5 mg)
Protocol tips
- For adsorption measurement, use 5 mM Tris pH 8.5 as diluent
Downstream tips
- Do not let the flow-through touch the column outlet after the second Buffer RA3 wash. Be sure to centrifuge at the corresponding speed for the respective time in order to remove ethanolic Buffer RA3 completely.

Publication protocol

The skin biopsies were immediately frozen in liquid nitrogen upon collection. They were stored at –80ºC for further processing. The frozen samples were homogenized by a PRO200 rotor-stator homogenizer (Pro Scientific) with Tri Reagent as the medium and frozen again at –20ºC. Following the phenol-chloroform extraction the transparent upper-phase samples were further purified and DNased using a NucleoSpin RNA XS kit (Macherey-Nagel).

RNA concentration of all samples was measured with a Qubit fluorometer (Invitrogen). The RNA quality of all samples was assessed by running a no-reverse-transcription negative real-time PCR with glyceraldehyde-3-phosphate dehydrogenase (GAPD) as the target sequence

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Skin using NucleoSpin® RNA XS from Macherey Nagel.

Paper title

Expression of selected genes of dendritic and Treg cells in blood and skin of morphea patients treated with UVA1 phototherapy

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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA XS below.

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