Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Add diluted Galacton-Star® substrate 1:50 with Reaction Buffer Diluent. Add 100 µL of Reaction Buffer, mix, and incubate for 30-90 min until light emission is maxima	Measure light signal in a luminometer for 0.1-1 sec/well.

Protocol tips
Add diluted Galacton-Star® substrate 1:50 with Reaction Buffer Diluent. Add 100 µL of Reaction Buffer, mix, and incubate for 30-90 min until light emission is maxima
Downstream tips
Measure light signal in a luminometer for 0.1-1 sec/well.

Publication protocol

BHK21 cells (ATCC: CLL-10) were grown in DMEM supplemented with 5% heat-inactivated FCS, 1 mM Na-pyruvate, 4 mM L-Gln, 10 mM HEPES and antibiotics. Cells were transfected with the indicated plasmids with Metafectene (Biontex, Germany) according to the manufacturer's instructions, cultured for an additional 24 hours. For luciferase or galactosidase enzyme activity cell lysates were analyzed by bioluminescence assays with Galacto-Star (Life Technologies) and Luciferase Reporter Assay kits (Promega), respectively. Signals were recorded on an Infinite-200 multireader (Tecan, Austria).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells below.

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