PureLink™ RNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required	- DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit

Protocol tips
- Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required
Downstream tips
- DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit

Publication protocol

Approximately 100 mg of sWAT was used for extraction of RNA using a pure link RNA isolation mini kit (Life Technologies, Carlsbad, CA). cDNA was synthesized and amplified using a high capacity RNA-cDNA kit and TaqManpre Amp master mix kit, respectively (Life Technologies, Carlsbad, CA). Quantitative real-time PCR analyses were performed on an ABI PRISM 7900HT using standard TaqMan master-mix reagents and gene-specific primer assays (Life Technologies, Carlsbad, CA). GAPDH was used as the housekeeping gene to normalize the expression of the target gene. sWAT from healthy children and adults was used as a control to calculate the delta CT values and the fold change.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink™ RNA Mini Kit below.

Download manufacturer protocol

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