RNeasy Fibrous Tissue Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit purifies RNA from up to 30 mg tissue		- If there is low RNA yield,Repeat RNA elution, incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - If there is low Low A260/A280 value use 10 mM Tris·Cl, pH 7.5, not RNase-free water, to dilute the sample before measuring purity. - If RNA does not perform well in downstream experiments this could be due to salt carryover dyuring elution step, so ensure that Buffer RPE is at 15–25°C.

Upstream tips
- This kit purifies RNA from up to 30 mg tissue
Downstream tips
- If there is low RNA yield,Repeat RNA elution, incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging. - If there is low Low A260/A280 value use 10 mM Tris·Cl, pH 7.5, not RNase-free water, to dilute the sample before measuring purity. - If RNA does not perform well in downstream experiments this could be due to salt carryover dyuring elution step, so ensure that Buffer RPE is at 15–25°C.

Publication protocol

The PDL tissues were homogenized using a homogenizer (Bullet Blender, Next Advance, NY, USA). The RNeasy Fibrous Mini kit (Qiagen) was then used to extract total RNA from the PDL tissues, according to the manufacturer's instructions. The extracted RNA was eluted in 25 µl of RNase-free sterile water (provided with the kit). The quality and concentration of RNA were determined by measuring the absorbance at a wavelength of 260 nm with the aid of a spectrophotometer (NanoDrop ND-1000, Thermo Scientific Inc, Rockford, IL, USA). The RNA samples used in this study had 260/280 nm ratios of ≥1.8. Half of isolated RNA was used for complementary DNA (cDNA) microarray experiments, and the other half was used for gene expression analysis with the quantitative reverse-transcription–polymerase chain reaction (qRT-PCR).

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Fibrous Tissue Mini Kit below.

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