PicoPure™ RNA Isolation Kit
RNA isolation / purification Tissue - Human testes
Publication protocol
Laser-dissected cells were captured in silicon-coated adhesive caps (Adhesive cap 500 opaque tube, Zeiss) and were lysed at 42°C in 10 µl of extraction buffer provided in the PicoPure RNA isolation kit (Arcturus). Cell lysates were stored at −80°C until further use. All procedures were performed under RNase-free conditions. Total RNA was isolated from cell lysates using the PicoPure RNA isolation kit (Arcturus) according to the manufacturer's protocol, including an on-column DNase treatment. RNA was eluted in 10 µl elution buffer. Subsequently, the RNA was concentrated to a volume of 5 µl with a speed vacuum centrifuge for 8 min. Total RNA isolated from 500 pooled cells was SPIA-amplified using the Ovation RNAseq V2 System (Nugen)Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Thermo Fisher Scientific for PicoPure™ RNA Isolation Kit below.
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