RNeasy Micro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit can be used for the isolation of RNA from up to 5 mg of tissue.		- Always blank the spectrophotometer with your elution buffer. - When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Upstream tips
- This kit can be used for the isolation of RNA from up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer. - When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

DNA was digested with RNase free DNase (Qiagen Inc, Valencia, CA) followed by RNA cleanup with the RNeasy Micro kit (Qiagen). One microgram of RNA was reverse-transcribed with Superscript III (Invitrogen). Products were amplified for 35 cycles with gene-specific primers utilizing RedTaq Ready Mix (Sigma-Aldrich, Saint Louis, MO). “No cDNA” samples were prepared for each primer set. DNA contamination was tested with control, intron-spanning primers. Products were resolved in a 2% agarose gel.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

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