Aurum™ Total RNA Fatty and Fibrous Tissue Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Before using lysis solution, add 500 ul of β-mercaptoethanol (β-ME) to the solution for a final concentration of 1%.	- To perform efficient elution preheat the elution solution at 70C in water bath priorto the elution step. - If there is high genomic DNA contamination, increase DNAse I digestion time and use only the DNAse dilution solution provided in the kit	- For better downstream application add appropriate volume of 95-100% ethanol to the wash solutions before intial use. - If elute volume is >80ul, there is a possibility of ethanol contamination. To reduce this, add 1-3min of centrifugation time after the final wash step.

Upstream tips
- Before using lysis solution, add 500 ul of β-mercaptoethanol (β-ME) to the solution for a final concentration of 1%.
Protocol tips
- To perform efficient elution preheat the elution solution at 70C in water bath priorto the elution step. - If there is high genomic DNA contamination, increase DNAse I digestion time and use only the DNAse dilution solution provided in the kit
Downstream tips
- For better downstream application add appropriate volume of 95-100% ethanol to the wash solutions before intial use. - If elute volume is >80ul, there is a possibility of ethanol contamination. To reduce this, add 1-3min of centrifugation time after the final wash step.

Publication protocol

Total RNA was isolated from myometrial biopsies by using the Aurum Total RNA Fatty and Fibrous Tissue Pack Kit (BioRad, Hercules, CA, USA) and reverse transcribed to generate cDNA by using the iScript cDNA Synthesis Kit (BioRad) according to the manufacturer's instructions. cDNA was stored at −20°C until PCR was performed. Forty-cycle PCR was performed on cDNA by using Taq PCR Master Mix (2X) (Affymetrix, Santa Clara, CA, USA) and primers (Table I) specific to NALCN, UNC80, UNC79, or NLF-1 (Integrated DNA Technologies, Coralville, IA, USA). Products were electrophoresed on a 1.5% agarose gel.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Uterus using Aurum™ Total RNA Fatty and Fibrous Tissue Kit from Bio-Rad Laboratories.

Manufacturer protocol

Download the product protocol from Bio-Rad Laboratories for Aurum™ Total RNA Fatty and Fibrous Tissue Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human Uterus using Aurum™ Total RNA Fatty and Fibrous Tissue Kit from Bio-Rad Laboratories. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.