RNeasy FFPE Kit

RNA isolation / purification Tissue - Human Uterus

Experiment

RNA isolation / purification Tissue - Human Uterus

Product

RNeasy FFPE Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- To allow complete penetration by formalin, use tissue samples less than 5 mm thick	- Perform all centrifugation steps using a microcentrifuge placed at 15–25°C	- Do not exceed fixation time of 24 hours as it results in poor performance in downstream assay

Upstream tips
- To allow complete penetration by formalin, use tissue samples less than 5 mm thick
Protocol tips
- Perform all centrifugation steps using a microcentrifuge placed at 15–25°C
Downstream tips
- Do not exceed fixation time of 24 hours as it results in poor performance in downstream assay

Publication protocol

Total RNA was extracted from untreated cells and cells after 3 h and 12 h of hormone treatment using RNeasy Mini Kits (Qiagen, Valencia, USA). Endometrial biopsies were collected from two patients (ages, 34 and 38 years) with unexplained infertility treated at the Nova Vita Clinic. Biopsies were collected on days LH+7 to LH+9 according to urine ovulation tests. Tissue samples were homogenized with Tissue lyzer (Qiagen) and total RNA was extracted from previously formalin-fixed (3.7%) endometrial biopsies using an RNeasy FFPE kit (Qiagen) according to the manufacturer’s instructions

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Reviews

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Uterus using RNeasy FFPE Kit from Qiagen.

Paper title

Changes in the Transcriptome of the Human Endometrial Ishikawa Cancer Cell Line Induced by Estrogen, Progesterone, Tamoxifen, and Mifepristone (RU486) as Detected by RNA-Sequencing

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy FFPE Kit below.

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Videos

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