miRNeasy Mini kit
RNA isolation / purification Tissue - Human Veins
Protocol tips
| Protocol tips |
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| - To capture a higher amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step. |
Publication protocol
RNA was isolated using the miRNeasy Mini Kit (Qiagen) and the Qiagen tissue lyser using 7 mm stainless steel beads. Tissue samples were disrupted for 5 min 30 Hz (1800 oscillations/min) in Qiazol lysis reagent. Further purification was done according to manufacturer's instructions. Concentration and purity was measured using NanoDrop 2000 (Thermo Scientific). RNA integrity was measured using Bioanalyzer RNA Nano Chip (Agilent). As expected for autopsy samples, the RNA integrity values (RIN) ranged between 1.8 and 2.7.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Qiagen for miRNeasy Mini kit below.
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