SV Total RNA Isolation System
RNA isolation / purification Tissue - Mouse Adrenal glands
Publication protocol
RNA extraction was performed using the SV Total RNA Isolation System according to the manufacturer's instructions (Promega). RNA quantification was performed with a NanoDrop spectrophotometer. The 260/280 absorbance ratio was used to asses RNA purity (mean ratio, 2025 ± 0.01). In addition, RNA integrity was assessed by running an aliquot on denaturing agarose gel stained with ethidium bromide. For cDNA synthesis, 500 ng of total RNA was reverse transcribed using a reverse transcription system (Promega).Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Manufacturer protocol
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