TRI Reagent® MRC

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA - The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Total RNA was extracted from 50 µL of blood using TRI Reagent BD technique (Molecular Research Centre, Inc.). [23] Murine tissue (0.5–2 mg) was homogenized using a bench-top homogenizer (Polytron® PT1600E; Kinematica AG, Littau-Luzem, Switzerland) in 1 ml TRIzol Reagent (Invitrogen, Carlsbad, CA). Total RNA was isolated from homogenized tissue using the RNeasy® Tissue Mini Kit (Qiagen) according to the manufacturer’s instructions. The miRNA concentration and purity were assessed by NanoDrop™ 1000 spectrophotometry (Nanodrop Technologies, Wilmington, DE, USA) and Agilent Bioanalyser (Agilent technologies, Germany).

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Manufacturer protocol

Download the product protocol from Molecular Research Center, Inc. for TRI Reagent® MRC below.

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Videos

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