Easy-Spin (DNA free) Total RNA Extraction Kit

RNA isolation / purification Tissue - Mouse Cornea

Experiment

RNA isolation / purification Tissue - Mouse Cornea

Product

Easy-Spin (DNA free) Total RNA Extraction Kit from iNtRON Biotechnology

Manufacturer

iNtRON Biotechnology

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- If lysate is allowed to overheating during homogenization, lysate can be placed on ice.	- For ethanol contamination, ensure that during the Washing buffer B, the easy-spinTM column is spun at maximum speed 1min to dry the easy-spinTM column

Protocol tips
- If lysate is allowed to overheating during homogenization, lysate can be placed on ice.
Downstream tips
- For ethanol contamination, ensure that during the Washing buffer B, the easy-spinTM column is spun at maximum speed 1min to dry the easy-spinTM column

Publication protocol

Total RNA was isolated from tissues using the Easy-Spin Total RNA extraction kit (GeneAll, Seoul, Korea). Following incubation with RNase-free DNase I (Promega, Madison, WI, USA), reverse transcription was performed using a random primer and MultiScribe™ MuLV reverse transcriptase (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions

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Reviews

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Cornea using Easy-Spin (DNA free) Total RNA Extraction Kit from iNtRON Biotechnology.

Paper title

Effects of nicotine on corneal wound healing following acute alkali burn

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Manufacturer protocol

Download the product protocol from iNtRON Biotechnology for Easy-Spin (DNA free) Total RNA Extraction Kit below.

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