|- If lysate is allowed to overheating during homogenization, lysate can be placed on ice.
|- For ethanol contamination, ensure that during the Washing buffer B, the easy-spinTM column is spun at maximum speed 1min to dry the easy-spinTM column
Easy-Spin (DNA free) Total RNA Extraction Kit from iNtRON Biotechnology has not yet been reviewed for this experiment
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2 years ago
Author: Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
3 years ago
Author: Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Download the product protocol from iNtRON Biotechnology for Easy-Spin (DNA free) Total RNA Extraction Kit below.Download manufacturer protocol
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